Rationale and perspectives on the success of vaccination against bovine herpesvirus-1. – PubMed

Indeed, we counted five fields of 100 cells each at 0, 24, 36, and 48 h posttransfection with pMASIA-tgD-VP22-YFP and observed that at 36 and 48 h, 45.6% ± 8.3% and 78.2% ± 11.3% of the transfected cells, respectively, were surrounded by cells with exclusive nuclear staining. The DNA vaccine pMASIA-tgD was complexed with increasing amounts of a stable synthetic analog of BNBD3 (aBNBD3) and then studied for its capacity to stimulate immune responses in mice in vivo. This created pMASIA-tgD-VP22, which encoded tgD fused in frame to VP22. Since the use of a BNBD as the cationic component in a peptide/DNA complexed BoHV-1 vaccine as a way to enhance humoral responses has not been studied, we evaluated the potential of this strategy. Vaccine brand used was determined to minimize recall bias and confusion with vaccines used to control other diseases. Several studies have reported a correlation between SN titers and protection against BVDV.

The migration is primarily initiated by a danger signal such as tumor necrosis factor alpha rather than by the antigen itself. There are many reports in the literature describing the development of recombinant BoHV-1 (rBoHV-1) as a vaccine vector (2, 33). Serum VN titers were measured on days 0-5 and 19. The companion diagnostic test detects antibodies against wild-type BHV1, whereas antibodies against the marker vaccine from which gE is deleted are not detected. All four vaccines induced increased BHV-1 antibodies by day 14 after the initial injection. The 107K protein was not glycosylated and induced antibodies that did not neutralize BHV-1.


In vitro studies showed that murine bone marrow-derived DCs (BMDCs) pretreated with aBNBD3 were activated, as evidenced by CD11c downregulation, and were functionally mature, as shown by increased allostimulatory ability. However, the effect of vitamin E oral administration to Japanese Black calves on antibody response to BHV-1 vaccination has not been elucidated. Heterogeneity in the biological properties was shown among the investigated recombinant viruses. These results demonstrated that PRV gIII and BHV-1 gIII share complementary functions. In another study, it has been shown that T lymphocyte homing to the genital mucosa requires the interaction of integrins αLβ2 and α4β1 with endothelial intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1), respectively [39, 40]. Calves were challenged IN with virulent BHV-1 on day 28 or 193 (seronegative calves) or day 105 (seropositive calves) after vaccination to evaluate vaccine efficacy.

The efficacy of the vaccine was tested after intranasal challenge of the calves with a BuHV-1 strain. However, it is known that among the 12 identified envelope glycoproteins, at least six (gC, gB, gD, gH, gK and gL) participate in viral attachment and entry. The TG of the eight vaccinates contained BHV-1 WT DNA, but in a statistically significantly lower amount than the unvaccinated controls. and Kaeberle, M.L., 1983. In addition to increasing the strength and kinetics of an immune response, adjuvants also play a role in determining the type of immune response generated. Risk for gE seroconversion significantly decreased over time for the HIGs, compared with the NIG.

Finally, 512 clinical serum samples from 3 major cattle-breeding provinces were monitored, and the overall positive rate was 34.57% (95% confidence interval: 30.45–38.69%). Eight separate, but related experiments, were carried out in which groups of six calves were vaccinated with one of eight commercial vaccines. As an approach to create a divalent DNA vaccine, a truncated secreted version of bovine herpesvirus-1 (BHV-1) glycoprotein D (tgD) and the amino-terminal subunit of glycoprotein B (gBb) were expressed from a dicistronic plasmid, designated pSLIAtgD-IRES-gBb. Bovine herpesvirus-1 (BoHV-1) causes significant disease in cattle including respiratory, fetal diseases, and reproductive tract infections. The aim of this study was to estimate the effect of the bovine herpesvirus 1 (BHV-1) vaccination on herd health and production in BHV-1 infected Estonian dairy cattle herds. To assess the serologic response of calves to inactivated and modified-live (ML) Mannheimia haemolytica (MH) preparations given alone and concurrently with combination viral vaccines containing ML bovine herpesvirus type 1 (BHV-1).

Bovine herpesvirus type 1 has two major immunogenic surface glycoproteins: a 90 kDa haemagglutinin and the 130 (74 + 54) kDa glycoprotein. The immunogenicity of bovine herpesvirus type 1 (BHV-1) hemagglutinin has been investigated. To assess the efficacy of a live glycoprotein E-negative bovine herpesvirus 1 (BHV1) vaccine to reduce transmission of BHV1 in cattle, a randomised, double-blind, placebo-controlled field trial including 84 herds was conducted in the Netherlands.