Quantitative Analysis of Herpesvirus Sequences from Normal Tissue and Fibropapillomas of Marine Turtles with Real-Time

This has been reported for the herpesvirus associated with lung-eye-trachea disease (LETD) in sea turtles, which has been shown to survive in seawater for about 2 weeks [51]. In addition, in this study we selected the sequence of the glycoprotein B (gB), gene as a tool to perform a higher resolution phylogenetic analysis on TeHV3 strains. How can you tell the difference between a pimple/ingrown hair and a herpes sore? The red eared sliders developed clinical signs and lesions consistent with those observed in the Burmese tortoises. Grey Patch Disease (GPD)-associated virus (Chelonid herpesvirus 1) affecting cultured green turtle hatchlings (Chelonia mydas) was reported to cause mortalities in 5-20% of the severely afflicted animals [59]. Identification of CFPHV deoxyribonucleic acid (DNA) in sea turtle tissues has been accomplished by various techniques, including PCR and in situ hybridization (Herbst et al.

For example, the bonobo genome was sequenced from a bonobo lymphoblastoid cell line which was immortalized in the laboratory using the human herpesvirus EBV (Prufer et al., 2012); this dataset is therefore much more likely to contain human EBV sequences, present in every cell, than primate gammaherpesviruses. The CPE segment provided a proven signal peptide for effective expression of type I membrane proteins in the baculovirus system followed by a furin cleavage site. Strain 1976 (passage 6) originated from the intestine of a Horsfield’s tortoise (Testudo horsfieldii) that died from TeHV-3 infection (18). Two previously described TeHV-3 strains were used. Because EmyHV-1 has been reported in the absence of lesions in the eastern river cooter,12 and transmission of a disease-associated herpesvirus between map turtles and painted turtles has been previously suggested,11 we were interested in investigating whether EmyHV-1 might be present subclinically in turtles that shared a water system with the dead map turtle. A nested PCR was carried out with 1 μl from the primary reaction as a template in a reaction mix and under the same conditions as above with 1 μM of each primer, TGV and LYG.

Jacobson ER. The pathology of TeHV3 has been thoroughly characterized in a transmission study in Greek tortoises infected with the type-strain 1976/98 with the virus inducing a disease whose severity was viral-load-dependent [34]. pp. Branch lengths show genetic distances in support of the classification of terrapeneHV2 as a novel herpesviral species. The aims of the present study were to characterize FP and detect CFPHV by histopathological and molecular analyses in tumors obtained from one loggerhead sea turtle caught at Boca da Barra, Cear�, Brazil. Presently, there are only two documented examples of challenge/vaccination experiments in reptiles [8], [9].

Divergent herpesviruses are more difficult to detect and assemble using traditional methods, mapping poorly to existing reference sequences (Pop, 2009). Given this, it is not surprising that CFPHV viral DNA sequences were detected in 100% of FP tumours. Virus isolation and quantification. X-rays of the head and perhaps the entire body can also help a vet determine what structures and organ systems are involved, including bones. Herpesviruses are known for their ability to establish lifelong infections. gB also has been extensively targeted for the development of many mammalian herpesvirus vaccines and for the development of serological assays for detection of herpesvirus infections (1, 5, 7, 13, 14).

UL24 family sequences were collected using exhaustive transitive PSI-BLAST (1) searches performed against the NCBI nonredundant protein database (posted 15 June 2005, 2,506,589 sequences) until profiles convergence (inclusion threshold, 0.01) and were filtered by identity. Herpesviruses have been implicated as the etiological agents of three marine turtle diseases. Indeed, these mutant viruses reconstituted from the newly constructed BACs grew well in cell cultures and could easily be detected, even in single infected cells, thanks to the red fluorescence emitted from the VP26-dsRed fused capsid protein. The current list is given in Table . Get a printable copy (PDF file) of the complete article (888K), or click on a page image below to browse page by page. Tumour cells were confirmed as fibroblasts or keratinocytes by positive staining with anti-vimentin and anti-pancytokeratin antibodies, respectively.

Within the nucleus, replication of viral DNA and transcription of viral genes occurs. The numbers of excluded residues are specified in square brackets. Visceral tumors, found in eight of 10 (80%) free-ranging turtles with cutaneous disease that were examined after death, had extensive interstitial fibrous proliferation.