Neurological disease in cattle in southern Brazil associated with Bovine herpesvirus infection. – PubMed

The disease is highly contagious, resulting in rapid spread of respiratory disease among cattle in close confinement, particularly in feedlots and when groups of cattle are transported. Twenty-two animals were necropsied. Often presents as respiratory disease but cows that had no respiratory signs can abort. Clinical signs vary from mild to severe balanoposthitis and may be associated with a decrease in semen quality. V. Primary infection is accompanied by various clinical manifestations such as infectious bovine rhinotracheitis, abortion, infectious pustular vulvovaginitis, and systemic infection in neonates.

Both viruses have common aspects in their pathogenesis: (1) they infect epithelial cells at the portal of entry and (2) they establish a latent infection in the sensory nerve ganglia, i.e., the trigeminal ganglia. Since their natural hosts are often kept in close contact with each other, concern was raised that a reservoir might be established in the heterologous host in addition to the homologous host. The complete long unique coding region (LUR) of BoHV-4 strain 66-p-347 was determined by a shotgun approach. It infects via mucosal epithelium, leading to rapid lytic replication and latent infection, primarily in sensory ganglia. In this report, we demonstrate that bovine herpesvirus 1 infection triggered tyrosine phosphorylation of proteins with molecular masses similar to those of phosphorylated viral structural proteins. Milk cells were tested with a nested PCR adjusted to detect the virus in the milk.

However, these developments have been impeded by the difficulty in manipulating its large genome using classical homologous recombination in eukaryotic cells. Reactivation from latency can occur after natural or corticosteroid-induced stress culminating in recurrent disease and/or virus transmission to uninfected animals. Some studies indicate that VP22 may enhance the transfer efficiency of therapeutic proteins by delivering them to more cells while trafficking. UL49 homologs are conserved among alphaherpesviruses. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. However, bovine herpesvirus 5 (BoHV5) is neurotropic and neuroinvasive and responsible for meningoencephalitis in cattle and in animal models.

Immunolabeling using VP8-specific antiserum and colloidal gold-labeled protein A as the electron-dense marker was used to identify VP8 in the virions and virus-infected cells. Six experiments were undertaken in which one or more quarters were infused with 1 ml. The absence of any antigenic or structural relationship between GVP 11a and GVP 11b, which were previously identified as one glycoprotein, GVP 11, demonstrated that these two GVP 11 species are unique glycoproteins. Since BHV-1 can induce apoptosis of cultured lymphocytes, we hypothesized that these virus-host interactions occur in cattle. It is a double-stranded DNA virus consisting of 33 structural proteins out of which 13 are associated with the envelope. After acute infection, the primary site for BHV-1 latency is sensory neurons in the trigeminal ganglia (TG).

Many factors make human contamination by BoHV-4 likely to occur. These characteristics include little or no pathogenicity, unlikely oncogenicity, the ability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, such as sheep, goats, swine, cats, dogs, rabbits, mink, horses, turkeys, ferrets, monkeys, hamsters, rats, mice, and chickens. We therefore performed an experiment that consisted of three phases. From these bulls, we collected a large panel of semen samples during 65 days postinfection (dpi). Previously we observed that live bovine herpesvirus-1 (BHV-1) induced considerable lymphocyte death in culture, suggesting that the virus infected one or more cell populations. In a rabbit seizure model, Us9-deleted BHV-5 failed to invade the central nervous system (CNS) following intranasal infection.

49 Holstein dairy cattle. The host range of BHV-4EGFPDeltaTK was characterized in vitro. A major problem with cattle uterine viral infections in general, and BoHV-4 in particular, is our limited understanding of the pathogenic role(s) that these infections play in the endometrium. Most disease is however, subclinical, hence its common name of “passenger virus”. Herpesvirus tegument protein VP22 can enhance the effect of therapeutic proteins in gene therapy, such as thymidine kinase (tk) and p53; however, the mechanism is unclear or controversial. Gammaherpesviruses are important pathogens in human and animal populations.

A study was conducted to determine the prevalence of bovine herpesvirus-1 (BHV-1), which causes infectious bovine rhinotracheitis, in cattle destined for market in Southern Province, Zambia.