Immunology and Cell Biology – Abstract of article: Bovine herpesvirus-1 vaccines

In summary, the ability of BHV-1 and HSV-1 to interfere with apoptosis and productive infection in sensory neurons is crucial for the life-long latency-reactivation cycle in their respective hosts. Links to PubMed are also available for Selected References. (Rhinomune, Pneumabort K), however, their efficacy sometimes is questionable. 1989. PrV gD and HSV-1 gD competed for binding to the V domain of HveC and both inhibited entry of the homologous and heterologous viruses. Effect of bovine alpha 1 interferon on bovine herpesvirus type 1 induced respiratory disease.

For this strategy to be successful, a fundamental understanding of virus-host interactions is required. In this study, we determined the ability of BHV-1 to replicate, cause cytopathic effects and affect cellular viability in a panel of normal, immortalized and transformed human cells from a variety of histological origins. BHV-5 is another bovine alphaherpesvirus, which causes fatal encephalitis in calves (7, 13). Five year serological study of bovine herpesvirus Type-1 in cattle in Lithuania. Kopp and T. Full text Full text is available as a scanned copy of the original print version.

AIl animals were successfully vaccinated as judged by the development of BHV-1 neutralising antibodies. This monoclonal antibody is produced as mouse ascites fluid, clarified by centrifugation, and filtered through a 0.2 micrometer filter. Isolates from clinical outbreaks up to 1995 were identified as a Cooper-like strain, while 2 of those in 1995 had characteristics of a “new” strain, which had never before been identified in Denmark or elsewhere in Europe. Three anti-apoptosis genes can be substituted for LAT, which will restore wild type levels of reactivation from latency to a LAT null mutant virus. Seventy-five percent and 91% of animals in groups 1 and 2, respectively, excreted BHV-5 in nasal and ocular discharges. In cell lines constitutively expressing both UL49.5 and gM, UL49.5 appears to be required for functional processing of gM.

Vaccinia-gIV-infected targets were lysed predominantly when PBMC were stimulated by fixed infected fibroblasts, whilst PBMC stimulated by UV-inactivated virus lysed mostly vaccinia-gIII-infected targets. Since the MAbs 3402 and R54 neutralize the BHV-1 and not BHV-5, it was presumed that these were important amino acids in defining the epitope. Consequently, we hypothesized that viral infection led to a novel form of cell death in RS cells compared to MDBK cells. Inhibition of phosphorylation of BHV-1 gE by tyrosine kinase inhibitors genistein and tyrphostin AG1478 substantially lowered the viral titer in Madin-Darby bovine kidney cells. For the evaluation of the amplifiability of the extracted nucleic acid from clinical specimens was included a bovine internal control that amplified a 626 bp fragment of the ND5 gene present in the bovine mitochondrial DNA. To gain a better understanding of the oncolytic breadth of BHV-1, the NCI panel of established human tumor cell lines was screened for sensitivity to the virus.

The highest mean virus titre was recorded for N569, followed by LA38 and 97/613. Mass spectrum (quadrupole time of flight [Q-TOF]) analyses of multimeric scFv demonstrated extensive heterogeneity due to differential cleavage, variable glycosylation (1 to 9 mannose residues), and the incorporation of minor unidentified adducts. One cell type that has received limited investigation for its role in the inflammation that accompanies BRD is the respiratory epithelial cell. Further, the virus was identified by agar gel immunodiffusion (AGID) test. We developed a polymerase chain reaction (PCR) assay to detect bovine herpesvirus type 1 (BHV-1) in bovine semen. Bovine herpes virus 1 establishes a latent infection in sensory ganglionic neurons of cattle.

The bICP0 protein encoded by bovine herpesvirus 1 stimulates productive infection and viral gene expression but inhibits interferon (IFN)-dependent transcription. Off-campus UNL users: To download campus access dissertations, please use the following link to log into our proxy server with your NU ID and password. The bovine herpesvirus 1 (BHV-1) US ORF8 protein with homology to the Us9 protein of other alphaherpesviruses induces apoptosis in rabbit kidney (RK13) cells without the presence of other BHV-1-encoded proteins. Tegument protein VP8 encoded by the UL47 gene of bovine herpesvirus type 1 (BHV-1) is the most abundant constituent of mature virions. Bovine herpesvirus 1 (BHV-1), like other members of theAlphaherpesvirinae subfamily, establishes latent infection in sensory neurons. While Bovine herpesvirus 1 (BoHV-1) has been known as a cause of bovine abortion for nearly 50 years, information is limited on the current prevalence of BoHV-1 abortion in the United Kingdom, or about the mode of virus dissemination to cause infection of the fetus.