Full Text Article: Immunity to bovine herpesvirus 1: I. Viral lifecycle and innate immunity.

The number of LR RNA-containing neurons in latently infected TG decreased significantly at 24 and 48 h PT but returned to near-normal levels by 72 h PT. We have identified stress-induced cellular transcription factors that are induced in sensory neurons during reaction from latency. Consequently, we predict that ORF2 expression is crucial for the latency reactivation cycle in cattle. Lytic cycle viral gene expression is detected within 6 h after dexamethasone treatment of calves latently infected with BHV-1. Although similar in many respects to the human immune response to human herpesvirus 1, the differences in the bovine virus proteins, immune system components and strategies, physiology, and lifestyle mean the bovine immune response to BHV-1 is unique. Lytic cycle viral gene expression is detected within 6 h after dexamethasone treatment of calves latently infected with BHV-1.

An LR mutant was constructed by inserting three stop codons near the beginning of the LR-RNA, suggesting that expression of LR proteins would be altered. haemolytica. Ata A., Kocamuftuoglu M., Hasiroioglu S. ORF2 inhibits apoptosis in transiently transfected cells, suggesting that it plays a crucial role in the latency-reactivation cycle. ORF2 inhibits apoptosis in transiently transfected cells, suggesting that it plays a crucial role in the latency-reactivation cycle. The symptoms of the related diseases are mainly non-life-threatening but have a rather wide host range that limits animal trade.


NIH, Mechanism of the Antiviral Activity of BAF Against Poxvirus and HSV-1 Infection. Journal of Virology 78: 4806–4816. Additional studies were performed to understand why ORF2 would interact with Notch3 and Notch1. Due to the lack of specific monoclonal antibodies (Mabs), the same recombinant glycoproteins were obtained from BoHV1 and used as positive control with a panel of specific gE and gI Mabs as well as in some ELISA assays. The isolates were confirmed by VNT and a conventional PCR. In 25 calves, the adrenal gland and at least 1 other tissue had lesions consistent with herpes viral infection.

In this study, a yeast 2-hybrid screen revealed that ORF2 interacted with two cellular transcription factors, Notch1 and Notch3. Administration of the adenovirus significantly inhibited thioglycollate-induced migration of polymorphonuclear leukocytes into the peritoneal cavity of BALB/c mice and reduced both clinical severity and articular damage in K/BxN serum transfer-induced arthritis. Lytic cycle viral gene expression is detected within 6 h after dexamethasone treatment of calves latently infected with BHV-1. The gross and microscopic findings were consistent with necrotizing meningoencephalitis caused by bovine herpesvirus (BHV-1 or BHV-5), and the diagnosis was confirmed by detection of bovine herpesviral antigen on fresh samples of brain via fluorescent antibody test using a monoclonal antibody against BHV-1 glycoprotein C. There are 8 known glycoproteins viz., gB, gC, gD, gF, gH, gI, gK and gL. A total of 50 μg of protein was separated in an 8% SDS-PAGE gel, and viral proteins were detected by Western blotting using antibodies specific for the designed BHV-1 genes.

Cats were euthanized on days 6, 10, and 30 post-inoculation. Administration of the adenovirus significantly inhibited thioglycollate-induced migration of polymorphonuclear leukocytes into the peritoneal cavity of BALB/c mice and reduced both clinical severity and articular damage in K/BxN serum transferinduced arthritis. Bovine herpesvirus 1 (BoHV–1) is the causative agent of a diverse clinical syndromes, including infectious bovine rhinotracheitis (IBR), infectious pustularvulvovaginitis (IPV) and infectious balanoposthitisabortion, infertility, conjunctivitis and encephalitis in bovine species (Gibbs and Rweyemamu, 1977). Moreover, BHV-1-infected MCF7 cells are significantly diminished in their capacity to form tumors in vivo. For example, two microRNAs (miRNAs) encoded within the LR gene are expressed in trigeminal ganglia of latently infected calves. An LR mutant virus containing stop codons at the amino terminus of ORF2 does not reactivate from latency or replicate efficiently in certain tissues, indicating that LR gene products are important.

Hence, herein, for the first time, we described the influences of TCDD on telomerase activity during virus infection. Three dexamethasone-induced transcription factors, Krüppel-like factor 15, Slug, and SPDEF, stimulated the herpes simplex virus type 1-infected cell protein 0 (ICP0) promoter more than 150-fold. Experiments designed to reveal the identity of the virion proteins capable of binding the RNA and to show whether the mRNA carried in the newly infected cells was expressed showed the following: (i) 32P-labeled riboprobe generated by in vitro transcription of the US8.5 ORF bound three proteins identified as the products of US11, UL47, and UL49 (VP22) genes.