Detection of Felis catus gammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic,

Results indicated 32%FcaGHV1seroprevalence, compared to 15%qPCR-evaluated prevalence (n=133);all but one qPCR positive animal was seropositive. Samples positive for DNA from any of three haemoplasma species had 19 times greater odds for testing positive for FcaGHV1 than haemoplasma negative cats in multivariable analyses after adjusting for age, sex and neuter status. Using an FcaGHV1-specific real-time quantitative PCR (qPCR) (1), we detected a high level of viral DNA (48 FcaGHV1 genomes per cell) in archived mesenteric lymph node DNA from a 9-year-old male cat with intestinal T cell lymphoma from Florida, USA (sample 31286). The odds for GHV infection were greater for FIV positive (OR = 4.5), male (OR = 13.32) and older (OR = 2.36) cats. Samples positive for DNA from any of three haemoplasma species had 19 times greater odds for testing positive for FcaGHV1 than haemoplasma negative cats in multivariable analyses after adjusting for age, sex and neuter status. Variation in whole blood load, up to 19,788 copies/10(6)cells, was detected in individual cats over time.

AIM • Investigate whether the prevalence of FcaGHV1 DNA detection and viral loads were different in normal cats compared with cats at risk of immunosuppression. Results indicated 32%FcaGHV1seroprevalence, compared to 15%qPCR-evaluated prevalence (n=133);all but one qPCR positive animal was seropositive. Risk factors for FcaGHV1 detection included being male, increasing age and coinfection with pathogenic retroviruses, feline immunodeficiency virus or feline leukaemia virus. Using an FcaGHV1-specific real-time quantitative PCR (qPCR) (1), we detected a high level of viral DNA (48 FcaGHV1 genomes per cell) in archived mesenteric lymph node DNA from a 9-year-old male cat with intestinal T cell lymphoma from Florida, USA (sample 31286). Domestic cats in the UK can be infected with FcaGHV1, confirming that this virus is globally endemic. Variation in whole blood load, up to 19,788 copies/106cells, was detected in individual cats over time.

FcaGHV1 is endemic in distant geographic regions and is associated with being sick and with coinfections. Samples positive for DNA from any of three haemoplasma species had 19 times greater odds for testing positive for FcaGHV1 than haemoplasma negative cats in multivariable analyses after adjusting for age, sex and neuter status. An FIV immunoflouresence test was developed as a positive control for transfection and assay function. FcaGHV1 was detected in 16% of domestic cats across all study sites. Risk factors for FcaGHV1 detection included being male, increasing age and coinfection with pathogenic retroviruses, feline immunodeficiency virus (FIV) or feline leukaemia virus. They decided to work on wild cats because they had a previous project in the lab funded by the National Science Foundation to study the ecology of diseases in domestic cats, bobcats, and puma, according to Troyer.

Beatty was awarded the Australian Small Animal Veterinary Association’s Award for Scientific Excellence in 2014 and a University of Sydney Thompson Fellowship in 2012. This review provides background information on progressive fibrosing lung disease in human and veterinary medicine and summarizes the evidence for an association between gammaherpesvirus infection and pulmonary fibrosis, especially Epstein-Barr virus in human pulmonary fibrosis, and equine herpesvirus 5 in equine multinodular pulmonary fibrosis. Inclusion in the booklet will “show case” exciting projects within the MBI community and facilitate linkage with other groups who share similar interests. FcaGHV1 was detected in 16% of domestic cats across all study sites. This review provides background information on progressive fibrosing lung disease in human and veterinary medicine and summarizes the evidence for an association between gammaherpesvirus infection and pulmonary fibrosis, especially Epstein-Barr virus in human pulmonary fibrosis, and equine herpesvirus 5 in equine multinodular pulmonary fibrosis. Dogs with cancers, including lymphoma, were more frequently positive than controls, but no particular association with B-cell lymphoma was noted.

However, indications of pathogen-covariance (potential facilitation) were also variably detected: potentially among FcaGHV1, Bartonella spp and Mycoplasma spp. The same symptoms may apply to diversely different infections, and pinpointing the exact cause for the condition can be complicated, since the feline urinary tract responds to various outside influences in a limited and predictable fashion. The gammaherpesvirus bovine herpesvirus-4 (BHV-4) has been isolated from a wide variety of animals, including lions and domestic cats. Methods: Polymerase chain reaction (PCR) assays and cytology were performed on 100 blood samples collected from Iranian Shorthair cats. Entries were classified according to body system treated, confirmation or suspicion of an abscess, evidence of microbiological evaluation being performed, any concurrent therapies given, and whether any reason was given for use of Convenia over alternative antimicrobials.